Student Projects
Masters Dissertations – 2019
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Project title: Study
of Heavy Metals from Sperata seenghala &
Clarias magur Fish from Pune District (Maharashtra),
India
Name of the student: Shubham Singh |
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From several decades
environmental pollution is considered as a
major global problem for both animals and
humans. The industrial effluents are the
major source of pollution that is discharged
into the water bodies posing serious threats
to the aquatic animals like fishes. If the
concentration of the heavy metal is not in
permissible limit as per World Health
Organization (WHO) guidelines then these
heavy metal accumulate in fishes and may
cause serious human health hazard. This
study was carried out to assess the
concentrations of various heavy metals from
the fish tissues of Sperata seenghala and
Clarius magur i.e; bones, muscles and liver.
The aim of this study was the investigation
of fish tissues contamination with heavy
metals. Samples were taken from the Bhor and
Pune fish market. Samples of tissues were
analysed for the concentration six heavy
metals (zinc, mercury, cadmium, iron,
nickel, and lead) using Inductively Coupled
Plasma Atomic Emission Spectroscopy (ICP-AES).
Iron and Zinc were most abundant in all fish
tissues. The level of in conclusion, it is
evident that even though the heavy metals
concentration in fish tissues of bhor and
pune region did not exceed the safe limit,
the continuous consumption of large
quantities of fishes can lead to metal
toxicity. |
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Project title:
Investigation of quantity & quality of
floral nectar
Name of the student: Vivek Pawar |
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Almost all flowering
plants produce nectar and quantity of the
nectar production varies as per the species
of the plant. Higher the quantity of the
nectar higher is the number of floral
visitors attracted towards the flowers.
Nectar is food and energy source of the
floral visitor. Floral nectar is widely
known as the key reward offered by
animal-pollinated plants to their pollen
vectors. These flower visitors primarily
include butterflies, beetles, ants etc. In
case of butterflies, number of studies has
shown that apart from the nectar quantity,
nectar composition, flower colour and
corolla tube length has significant impact
on the number as well as types of butterfly
visitors a flower would get. Amboli was
selected as study area. Amboli region is
situated at Sawantwadi tehsil in the
Sindudurg District of Maharashtra, India. It
is 350 km away from Pune city. Amboli is
located near Ajara-Sawantwadi road and is
rich in flora and fauna. During study period
nectar quantity was assessed in 22 species
of flowers. The quantity was found in the
range of 0.29 μL /flower to 12.01 μL. The
variation can be attributed to multiple
factors such as type of species and flower
size. In case of bagging and without bagging
species, without bagging species shows
variety in their amount of nectar secretion
or production. To check the floral nectar
composition, sugar composition was studied
using FTIR. It is not a quantitative
technique but it shows qualitative
variations. Nectars from 14 species were
analysed. Variation in carbohydrates
quantity was seen. In all the floral nectar
composition sucrose, glucose and fructose
sugars are present but its concentration
varies. Nectar collection time, floral
origin, geographic location, possible
environmental pollution, temperature and
humidity may affect the nectar composition
and concentration. For proboscis length and
corolla tube measurement we measured
proboscis length of 80 individuals in 10
different butterfly species as well as
corolla tube length of 6 plant species to
find out possible correlation between
proboscis length and corolla tube
measurement . In this study it was observed
that there is no correlation between corolla
tube and proboscis length, it was verified
using Bartlett followed by ANOVA. Similarly
in other case to understand the correlation
between flower colour and their visitors we
recorded the flower colour of 15 plant
species and their visitors. It was observed
that there was no correlation between floral
visitors and flower colour, using Bartlett
followed by ANOVA. |
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Project Title: Seed
size in relation to seed moisture content
and germination success
Name of the student: Alap Bhatt |
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The Spermatophytes are
endowed with this peculiar but very common
detachable vehicle of reproduction carrying
the germ of a new individual which is known
as the Seed. It is product of sexual
reproduction that consists of endosperm,
embryo and seed coat. Seed is responsible
for species establishment and growth form.
It is important to study on ecological role
of seed in forest habitat. 16 woody plant
species studied to understand seed size
relation with germination success, canopy
effect on germination and soil fertility
association with seed germination. Present
study was carried out at Amboli forest,
Northern Western Ghats, Maharashtra. Out of
16 species 2 species are endemic to Western
Ghats and 2 species are listed in IUCN red
list. In these studies we observed strong
correlation between seed size and canopy
percent in germination success. Hypothesis
tested was large seeded species have less
germination success in open area. It is true
in our studies, Mann Whitney test (100<75)
we accept the hypothesis. In other
hypothesis large seeded species associate
with low soil fertility. We observed higher
concentration of N, P, K and OC in canopy
site compare to open site. As we found large
seeded species have less germination success
in open area, we can reject the second
hypothesis. In against us observed small
seeded species associated with low soil
fertility. We found weak relation (r2 =
0.1562) between seed moisture content and
germination success. |
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Project Title:
Effects of environmental and geographical
correlates on species assemblages of
threatened and endemic anuran genera of
Western Ghats
Name of the student: Anusua Pal |
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THE WESTERN GHATS of
the India harbour unique assemblages of
ancient endemic animal and plant taxa, by
the virtue of geological and climatic
changes that happened for around 65 my. The
rich geological history of this region has
shaped characteristic geography of this
region which in turn has affected the
evolution of various organisms.
Specifically, the geographical
discontinuities in the Western Ghats such as
the Shencottah Gap, Palghat Gap and Goa gap
have affected the dispersal and vicariance
in Anurans. This has affected the species
compositions and community structure of
amphibians in the regions around these gaps.
The species composition
is further affected by current environmental
conditions which are also reflected in
species composition. Physical conditions of
an organism's environment like temperature,
light, moisture and the food resources it
contains primarily determine the
distribution of the organism in both spatial
and temporal scale. These environmental
factors are unevenly distributed and hence
cause uneven distribution of species.
Here, I attempt to find such effects of
geographical distance, elevation and
environmental variables such as annual
precipitation and temperature range, on the
species assemblages of three endemic genera
of Western Ghats namely, Nyctibatrachus,
Micrixalus and Indirana. Five major regions
of the Western Ghats pertaining to their
geological origins and/or geographical gaps
between them were considered. The presence
only data for the species of Nyctibatrachus,
Micrixalus and Indirana was extracted from
articles describing the species for the
first time, defining the neotype based on
the original description and/or studies
relying on molecular identification for the
confirmation of the species. The linear
discriminant analysis (LDA) was performed to
understand if the defined regions show any
difference in their environmental
structuring. The beta partition analysis was
run in R to find out the process of species
turnover shaping the species assemblages
between regions. The zeta diversity analysis
was implied to understand extent of zeta
diversity decline, the effective distance
for zeta diversity decline and effect of
geographical distance, elevation and
environmental variables on the structure of
species assemblages.
The discriminant analysis resulted in
largely overlapping clusters of regions with
only distinct partitioning between the
regions below and above the Nilgiri
elevational regime. Although, the regions
were largely homogenous, the species
assemblages between the regions showed very
low nestedness. Further, zeta decline
analysis also showed the sharp trend of zeta
diversity decline indicating the low
nestedness. However, the zeta diversity
decline was gradual for Indirana and
sharpest for Nyctibatrachus indicating that
the species sharing between sites for
Indirana was more than two other genera. The
high retention rate for Indirana also points
towards the fact that the genus comprises of
more generalist species than other two
genera. However, none of the genera showed
species retention beyond four sites. The
multi-site generalised dissimilarity
modelling showed geographical distance
between the two (zeta order 2) sites as most
effective factor for defining the species
assemblages in these genera. For the genus
Indirana; elevation, temperature annual
range, and precipitation of coldest quarter
were also effective factors for determining
species assemblages between sites. For the
genus Micrixalus annual precipitation was
shown to be additional factor defining
species assemblages. While, for the genus
Nyctibatrachus annual temperature range,
annual precipitation and precipitation of
coldest quarter were additionally shown to
be effective. The species assemblage
structure between 3 sites was shown to be
affected by Human Influence Index in
Indirana. The results of these analyses
effectively show that these genera have high
composition of rare species. The species
assemblages of one site are seldom repeated
up to 4 sites indicating the site
specificity of the species of these genera.
The nestedness being very low and extending
only between two to three sites makes it
crucial to conserve the natural habitats
which are in close vicinity, otherwise
threatening the rare species composition. In
the era where human activities are
threatening the natural habitats and
changing the local and global climate and
or/weather (the Anthropocene) the studies
like the one presented here become
pertinent.
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Project Title:
Investigating the Spatiotemporal Variation
of Chemical Signals in Musa Plants
Name of the student: Aparna Sakpal |
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Banana (Musa sp.) ranks
fourth in terms of worldwide fruit
production, and has high economical and
nutritional value. Amongst the factors that
contribute to the lower productivity in
banana crops, abiotic stress factors like
scarcity of water, soil salinity and extreme
temperatures are the most significant.
Hence, the present study attempts to
understand the effects of variations in the
environmental factors on the Musa
phytochemical concentrations, compounds
which are known as stress indicators in
plants. To understand if there was any
variation in the chemical signals, plants
were planted in polyhouse and field of
Fursungi. The morphological, physiological
and biochemical estimations were studied in
Musa acuminata (Grand Nain) variety leaves
through its vegetative developmental stage.
We have quantitatively estimated the amounts
of antioxidants, proline, and total phenols
in banana and correlated it with the abiotic
factors, along with the amounts of total
chlorophylls; to understand the variations
in their concentrations on the basis of the
leaf loci on the pseudostem, developmental
stage and their growth conditions. We found
significant variations in the stress related
phytochemical concentrations at different
leaf positions on the same individual,
indicating the variations in the effects of
abiotic factors on different plant parts and
their response with respect to their age. |
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Project Title:
Effect of salt Stress along with hormones
and Acinetobacter on different varieties of
Chickpea (Cicer arietinum L.)
Name of the student: Ashwini Bhande |
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A laboratory experiment
was conducted at botany department,
Savitribai Phule Pune university, pune, to
evaluate the effect of different
concentrations of salt, hormones and
acinetobacter on two Chickpea cultivars viz;
(Phule-G-9425-9 and Phule-G-9425-5), which
were obtained from Mahatma Phule Krishi
Vidyapeeth, Rahuri, Ahmednagar. Sodium
Chloride used as a source of graded levels
of salinity. Naphthalene acetic acid (NAA)
and Indole butyric acid (IBA) was used as a
plant growth hormones and Acinetobacter
tandoii spp. was used as a bacterial
culture. The salinity levels imposed were-
Control 4dS/m-1, 6dS/m-1, 8dS/m-1, 10dS/m-1,
12dS/m-1 and 14dS/m-1 EC were tested using
Conductivity meter. The different
concentrations of growth hormones were
prepared in serially diluted between the
range of 10-1 and 10-5. Bacterial culture (Acinetobacter
spp.) suspension was incubated for 3 days
and different concentrations were prepared
using method of serial dilution.
The study showed that
germination percentage and seed vigor index
significantly decreased with increase in
salt concentration. Phule-G-9425-9 showed
better performance than Phule-G-9425-5 in
terms of germination percentage and seed
vigor index. Also in biochemical test,
Catalase and Superoxide dismutase (SOD)
enzyme activity were increased with increase
in salt concentration. The chlorophyll
content of both chickpea cultivars were
decreased with increase in salt
concentration, protein content were
decreased and proline content were increased
with increase in salt concentration.
As compared with growth hormones and
Acinetobacter, Acinetobacter spp. showed
better results than growth hormones. Also
compared with growth hormones Naphthalene
acetic acid (NAA) 10-5 showed good results
than Indole butyric acid (IAA)10-4. |
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Project Title:
Isolation, Characterization and
Identification of Plant Growth Promoting
Rhizobacteria (PGPR) and their Effect on
Seed Germination
Name of the student: Kartiki Kane |
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Soil is rich in
microflora which includes Plant Growth
Promoting Rhizobacteria (PGPR). PGPR are
those bacteria that colonize the rhizosphere
soil. Plant-microbe interactions are
required in order to maintain good plant
health. This project mainly focuses on the
role of PGPR in enhancing the germination
process of seeds. Here, we have isolated
PGPR from invasive plant species like
Lantana camara and Parthenium spp. These two
species were chosen due to their high
tolerance levels towards abiotic stress (Stefania
Toscano et al. 2019). The isolates have been
identified as Bacillus cereus, Bacillus
licheniformis and Azotobacter spp. They were
further characterized for their plant growth
promoting traits which included tests like
Indole-3-Acetic acid (IAA), Phosphate
solubilisation, Nitrogen fixation and Starch
hydrolysis. A consortium of these bacteria
was made and tested on crop seeds which
included Fenugreek, Pea and Moth bean.
Trials showed the effect of PGPR consortium
on these seeds. Different proportions of
this consortium with a fixed absorbance of 1
were tested on the seeds. Each set consisted
of control (10 seeds) and 9 test sets (10
seeds) treated with different proportions of
PGPR consortium. The consortium proportions
are as follows: (Bacillus cereus: Bacillus
licheniformis: Azotobacter spp.) 1:1:1,
1:0.5:0.5, 1:0.25:0.25, 0.5:1:0.5,
0.5:0.5:0.25, 0.5:0.25:1, 0.25:1:0.25,
0.25:0.5:1, 0.25:0.25:0.5. Consortium in the
proportion 1:1:1 (Bacillus cereus: Bacillus
licheniformis: Azotobacter spp.) was found
to be most effective on seed germination and
seedling health. Therefore, these PGPRs
isolated from invasive weeds can be used as
inoculants in agriculture in the form of bio
fertilizers. |
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Project Title: Study
on Roadkill of animal in Amboli region,
Sindhudurg
Name of the student: Pradhumansinh Sindha |
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The diversity and
abundance of the animals which are killed in
animal-vehicle collision (roadkills) were
studied along with the traffic load, in
Amboli forest, Sindhudurg district; a
popular tourist destination in Maharashtra.
Systematic surveys to collect data on road
kills and count of vehicles were conducted
along four roads heading towards popular
‘tourist points’ of Amboli. Over 90 km of
road surveys revealed 138 individuals of
road kills belonging to 32 species. The road
kills included animals from little Indian
field mice to wild boar. Out of 138 road
killed animals, reptiles represented 61%,
amphibians 33%, mammals 7 %, birds 2 %.
Endemism was also found to be high among the
road killed animals. Of 32 species, 10
species were endemic to Western Ghats or
India. I also tried to explore the relation
among vehicle load and number of animals
killed due to vehicle collision in
pre-monsoon (55%) and monsoon (33%) season
and in winter (12%) mortality of bird is
observed. The relation of body size and road
kill number shows less body size was
associated with high no of road kill
incidence. Setting speed limit, public
awareness, putting up of informative boards,
keeping an upper limit on the entry of
vehicles in Amboli are among some of the
mitigation measures to potentially reduce
the road kill incidences and in turn
conserve biological diversity. |
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Project Title:
Diversity of Pseudoscorpions in western
Maharashtra, northern Karnataka and Goa
Name of the student: Harshita Rana |
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Pseudoscorpions are a
small group of Arachnids which belong to the
Order Chelonethi. They are cosmopolitan and
are distributed over a wide range of
habitats, and are also known to occupy an
important position as predators among the
bark and litter fauna, feeding on a variety
of insects. Despite their diversity of form
and habitats, the Pseudoscorpion fauna of
India is poorly known, in part because of
lack of the taxonomic resources. This study
aimed to study the diversity of
Pseudoscorpions from Western Maharashtra,
North Karnataka and Goa and provide the
missing taxonomic aids for further studies
on the Indian fauna of the group. An
elaborative and illustrated morphology is
thus provided, along with detailed
descriptions of the taxonomically important
characters. These descriptions are further
used for development of a classification
system for the pseudoscopions of the Indian
fauna within the range of the selected study
area, that is, Western Maharashtra, Northern
Karnataka and Goa. The taxonomic key was
developed by updating or modifying the
existing key available, in the work done by
Murthy and Ananthakrishnan 1977, which is
the only available literature on the subject
from the Indian Fauna. The pseudoscorpion
fauna of the world includes 27 families, and
that of India includes 12 families out of
which 6 families are recorded and reported
from the selected study areas, in the
current study. It is believed that this
study would stimulate further work on this
important group of Arachnids, more so their
phylogeny, biology or ecology. |
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Project title:
Effect of Root Leachates and Bacterial
Endophytes Isolated from Cassia tora and
Chromolaena Odorata roots on Vigna Radiata
Germination
Name of the student: Ishwari Latey |
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The Endophytic bacteria
which colonize the internal root tissues are
an important part of plant microbiota. They
establish various relationships with the
host, sans parasitism. The biodiversity of
endophytic bacteria in weeds has been
significantly studied in the light of plant
growth promotion activity and their stress
tolerant capability. However, behind the
prolific spreading and successful survival
of weeds in the environment, allelopathic
mechanism is also involved. The major
studies are done on allelopathic potential
of fungi and their role in host plant’s
successful survival and prolific breeding.
In this study, we aim to answer whether the
endophytic bacteria play any role in
allelochemical defence exhibited by host
plant. We have studied the endophytes
isolated from the roots of Cassia tora and
Chromolaena odorata, two widespread exotic
weeds in the Western Ghats.The selected
bacteria were identified by standard
procedures as Serratia marcescens subsp.
Sakuensis, Serratia liquefaciens, Bacillus
sp. and Staphylococcus sp. These bacteria
were used for carrying out seed germination
studies. The experiment was designed such as
to check the seed germination ability in
controlled variables (germination paper
plate test) as well as natural, uncontrolled
variables (pot test). The isolated
microorganisms were screened on the basis of
plant growth promoting and antagonistic
hormones, Indole-3-acetic acid and Hydrogen
cyanide, respectively. To study the effect
of concentration of bacteria on seedling
germination, the selected bacteria were
applied on the test seeds in 10-1 to 10-5
dilutions and growth observation were
recorded. Statistical analysis of the
observations (with p<0.01) showed that the
plant growth parameters are mostly bacterial
concentration dependent with respect to the
control, proving that the plant growth
promoting role of isolated bacteria is
associated with a particular bacterial
concentration. Secondary metabolites of
plants play an important role in growth
other plants and microbes in the vicinity.
The effect of secondary metabolites of
Cassia tora and Chromolaena odorata was
observed by applying root leachates of
concentrations 5% ,10% and 15% on test seeds
using germination paper plate test and the
observations were recorded. The root
leachates showed significant differences in
growth with respect to the control. Thus in
this study we have tried to enlighten the
role of endophytic bacteria in plant’s
defence mechanism, improving the
understanding of plant. |
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Project title:
Nesting Habitat Characterization of White
Bellied Sea Eagle (Haliaeetus leucogaster)
in the Raigad District area
Name of the student: Manashree Bapat |
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White bellied sea eagle
(Haliaeetus leucogaster) is an indicator
species to measure biodiversity, general
health of the ecosystem and is present at
the highest level of coastal food chain.
Considered to have a declining population,
concern has been expressed in recent years
over the conservation status of this eagle.
To investigate current nesting distribution
of the species in Raigad district, we
conducted field survey method to collect
regional data as it is important criteria to
study its habitat selection. Data were
obtained by surveying around 150 km of
coastline. We found 22 nests of which 13
nests found in Shrivardhan tehsil and rest
in Alibag, Murud tehsil. We collected data
on their nesting parameters like tree
preference, height from ground level,
distance from seashore etc. We observed
around 59% nests on Casuarina equistifolia,
and rest on Sterculia foetida, Mangifera
indica, Ficus bengalensis etc. Based on our
observations and information by local
birdwatchers, the number of indigenous trees
like Sterculia foetida is decreasing, there
has been a shift in preference for nesting
habit from such indigenous trees to exotic
species like Casuarina equistifolia. The
coastal areas of Alibag, Murud, Shrivardhan
tehsil are highly disturbed because of
rapidly increasing tourism and urbanization.
As per our observations and statistical
analysis human disturbance is adversely
affecting the nesting habitat of this eagle.
So, we conclude that the white bellied sea
eagle is under threat and there is need to
study various aspects related to this
species and its populations for its
conservation. |
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Project title: Study
of Gastropod Shell Preferences in Diogenes
Chhapgari Trivedi, Osawa & Vachhrajani
Name of the student: Neeyati Limje |
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Diogenes chhapgari
Trivedi, Osawa & Vachhrajani, 2016 is newly
described species of hermit crab from
coastal areas of Gulf of Khambhat, Gujarat
and very little is known about its ecology.
The present study is a first approach to
study the ecological aspect of the species.
Sampling for the collection of the species
was carried out using hand picking method
from October to December, 2018 in the
mudflat habitat of Dandi, Gujarat and
Mumbai, Maharashtra. The specimens were
identified up to species level and
categorized in three groups’ viz male,
female and ovigerous female. The crab
specimens were weighed and their size
(shield length) was measured. The gastropod
shells were identified using standard
literature and keys and different
morphological characters were measured.
Total 1437 individuals of hermit crab were
captured (1086 males, 243 females and 108
ovigerous females), occupying 13 different
species of gastropod shells. Amongst all the
shell species identified, Cantharus spiralis
was highly occupied by the crab species
followed by Semiricinula tissoti, Nassarius
stolatus, Latirus nassoides, and Umbonium
vestiarium. D. chhapgari males were
significantly larger in size than the female
and ovigerous females. Body size and weight
of the hermit crab showed significant
Correlation with different morphological
characters of gastropod shell. Strong
correlation was recorded between crab body
size and shell dry weight which shows shell
weight has significant impact on shell
selection pattern of hermit crab. |
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Project Title:
Exploration of Fecal Endoparasites in
Herbivore Population in Saswad Area, Pune
Name of the student: Nikhila Purohit |
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Surveying and
identifying internal parasites is important
for maintaining overall health of the
animal. Parasites can potentially affect the
population growth of a species as well as
interactions between species. Endoparasitic
study plays a major role in population
regulation. This study is conducted in
Saswad-Waghapur Grassland, 40 Km away from
Pune City. Present study aims to assess the
endoparasitic load and diversity of domestic
herbivore (goat, sheep) and wild herbivore
(Indian gazelle) across two sites in Saswad
area. Goat, sheep and Indian gazelle in this
area shares the same grazing land, there is
a chance to spread endoparasitic infection
between these herbivores. A non-invasive
method was opted for analysis of
endoparasites of collected fecal samples.
Total 104 fresh fecal samples collected from
2 study areas in Saswad. 58 samples from
Pargaon village and 46 samples from Aamble
village were collected during September
2018-March 2019. Collected fecal samples
were analyzed by the Zinc Sulfate
Centrifugation method. Through the analysis,
Eimeria spp. (Goat,N=179;Indian gazelle,N=74)
and Coccidia spp. (Goat,N=594;Indian
gazelle,N=226) were most abundant in both
herbivore species. High number of
endoparases found in goat and sheep than
Indian gazelle. It observed that some
parasites, Strongyloid spp., Ascaris spp.,
and some unidentified species of
endoparasite also infecting these
herbivores. More fecal samples will signify
the spreading pattern of endoparasites. This
study provides baseline data for further
research on wild and domestic herbivore. The
data generated through this study will prove
useful for researchers and conservationist
regarding the health management of these
species. |
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Project title:
Investigating Aflatoxin Degradation using
GRAS (Generally Recognized As Safe)
Organisms
Name of the student: Prafulla Vaidya |
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Contamination of food
and feed by aflatoxin (AF) is a grave
problem as one cannot control the ingestion
of the toxin, this cause a serious economic
and health problem. Of the different
processes to degrade the toxins is well
known, degradation by biological methods
utilizing Generally Regarded As Safe
Organism (GRAS) namely Saccharomyces
cerevisiae and Lactobacillus casei isolated
from YakultTM were used in this study.
Aflatoxin production by Aspergillus flavus
was isolated from an infected groundnut.
Further degradation was checked by the GRAS
organisms through cell binding method. These
organisms are known to have an ability to
bind the aflatoxin on their cell wall and
thus the reduce the concentration of
aflatoxin, to such a level which are not
toxic when consumed. An initial of 20μg/mL
of AFB1 was used in the study. The amount of
AFB1 remaining was measured against time by
thin layer chromatography (TLC) and UV
spectrometry. Both the species were able to
degrade AFB1, and no significant difference
was found between them. However,
Saccharomyces cerevisiae was found to
degrade 42% of the toxin in first two hours
and reached to 53 % in 12 hours.
Lactobacillus casei degraded about 16% 4
hours and reached the degradation to 23% in
13 hours. To the best of our knowledge this
is the first study carried out to determine
at which growth phase of the organism are
the toxin degraded. This study may further
open new avenues to understand the
metabolomics in the lag phase to degrade the
toxin. |
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Project title:
Studying effect of colchicine on Hydra
Name of the student: Pratik Paranjape |
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Interstitial stem cells
in hydra are multipotent cells which
continuously differentiate into nerve cells,
nematocytes, cnidoblasts and gland cells of
hydra. Colchicine is a chemical which
inhibits cell cycle by arresting the cells
at metaphase of the mitosis. In hydra cell
counting can be done by macerating and
making a smear after staining with reduced
methylene blue. The smear was observed under
40x in microscope, photographed and the
cells were counted easily. Comparing the
cells of colchicine treatment for 24 hour
and 48 hour with the control hydra gives the
difference between the numbers of cnidoblast.
The total number of cells present in each of
the sets is related to the action of
colchicine, so by taking cell count of these
colchicine treated hydra we can predict the
action. |
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Project title:
Variation in Bird Diversity with Habitat
Quality in Panvel, Maharashtra
Name of the student: Rahul Misal |
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As urbanization is
increasing throughout the world it has an
impact on wild life. Avian species are used
and act as an indicator to analyze the
impact in the surroundings. This study aims
to understand the urban impacts on bird such
has quality and quantity of vegetation,
human disturbance across the urban areas to
the urban fringes (remnant parts) of Panvel
City. Its shows increase in urbanization
give rise to decrease in species richness of
birds and decrease in vegetation percentage.
Remnant parts namely Gadeshwar and Shivansai
shows high species richness as they are
situated far away from the City area and
situated near foot hills of Matheran which
falls under Western Ghats of Maharasthtra,
where as the Urban area shows the low
species richness of birds due to high human
disturbance and low vegetation quantity and
quality. |
|
Project title:
Diversity and Distribution of Various
Species of Geckos of Northern Western Ghats
Name of the student: Saurabh Swami |
|
The geckos are the most
diverse and distributed group of Reptiles
all over the world. They inhabit a wide
array of habitats including tropical and
temperate forests, mountains, deserts,
swamps, grassland, farmland, even shrubs and
are also found in abundance in cities.
Irrespective of their widespread
distribution and relative abundance, very
little data is available on distribution of
geckos. In India, the geckos are widely
distributed across all the landscapes. Some
of the geckos are known to be endemic to
Western Ghats. Although, many new species of
geckos are being discovered recently, yet
very less literature is available on studies
on their distribution and abundance. The
distribution patterns of geckos are well
understood by knowing their habitat
preferences. The diversity indices can be
used to know and study their important
habitats, which are the habitats showing
maximum diversity and species dominance.
Seven different species of geckos (Hemidactylus
maculatus, Hemidactylus murrayi,Hemidactylus
prashadi, Hemidactylus sahagali,
Cyrtodactylus albofasciatus, Cyrtodactylus
deccanensis, Eublepharis fuscus) were
reported from five different localities (Matheran,
Tamhini Ghat, Malhargad, Chalkewadi, Amboli). |
|
Project title: Cultivated traditional rice
varieties and preference towards them in
Jawhar tehsil, District Palghar
Name of the student: Neehar Barve |
|
Palghar is a
relatively new district. The Jawhar tehsil
of this district has significant amount of
tribal population and with them their
indigenous and traditional rice varieties.
Rice varieties are a large part in India’s
Agrobiodiversity which needs urgent efforts
for conservation. The study was carried out
understand and know the traditional rice
varieties in cultivation and the rice
cultivator’s preference towards them. Five
villages namely Gardwadi, Kelicha pada,
Ramnagar and Adkhadak were sample for 20
cultivators each with total of 80
cultivators. The cultivators were surveyed
for their personal & socio-economic profile,
traditional rice variety they cultivate and
their preference towards them and lastly
their constraint with respect to traditional
rice cultivation. The findings of this study
include association between personal &
socio-economic profile and the preference
towards traditional rice varieties, major
constraints like low market prices and lack
of knowledge and awareness about rice
varieties and traditional cultivation
practices. |
|
Project title:
Investigating diet of Indian grey wolf (Canis
lupus pallipes) in Saswad grassland, Pune
Name of the student: Unmesh Mitra |
|
Indian Grey wolf (Canis
lupus pallipes) was historically widespread
throughout Indian Sub-continent. Today it is
an endangered species in Schedule I of the
Wild Life (Protection) Act, 1972 in India.
It has been seen worldwide that if the
habitats have high anthropogenic pressure
with low abundance of wild prey, the wolves
feed on livestock which may lead to
human-wildlife conflict Therefore, it was
necessary to estimate their dependency on
livestock in human dominated landscape. This
study is conducted in Saswad-Waghapur
Grassland, 40 Km away from Pune City. Scat
analysis method was used to identify the
prey base of wolf. This study was conducted
on scats from two adjacent wolf packs, 3
individuals in each pack. Samples were
collected between June 2018 to January 2019
in Saswad-Waghapur grassland. Majority of
scats (71.4%) contain single prey item,
28.57% scats contain double prey item
whether 0.03% scats contain 3 prey items.
68% of wolf’s diet consist of livestock, 27%
is wild prey and rest 5% is unidentified. In
livestock, Goat is the most consumed prey
(49%) in wolf diet. While rodents (67%) are
the most abundant wild prey found in wolf’s
diet. It is a challenge for the managers to
reduce dependency of wolf on domestic
livestock to minimize Human-Wolf conflict.
The survival of wolf is important for the
stability & functioning of the entire prey
base also important for existence of healthy
and vigorous prey populations. |
|
Project title:
Variation in bio-acoustic communication of
free ranging Indian Peafowl (Pavo cristatus)
Population
Name of the student: Vedanti Mahimkar |
|
Communication is an
integral part of animal behaviour. Birds
emit vocal signals in response to different
events. These vocal signals play an
important role in sociobiology of birds
since every signal conveys different
information or message. Previous studies
have been aimed at studying acoustic
communications among oscine birds. However,
very little is known about the acoustic
communication patterns and physical
characteristics of calls of non-oscine
birds. To overcome this research gap and
gain new insights on non-oscine bird
communications, we aim to study, the
acoustic communication of free-ranging
Indian Peafowl (Pavo cristatus) population.
The study was conducted from May 2018 till
December 2018 in Pune, Maharashtra, India.
We have addressed,
The vocal repertoire of free-ranging
Indian Peacock
Characteristics of vocal signal and
variation in it
The behavior(s) associated with these vocal
signals
The characterization and analysis of
these acoustic signals was done with
computer-assisted sound analysis. Different
call types were identified by studying
spectrogram. Each call was assessed for the
total number of notes, call duration,
duration of each note, duration between two
successive notes and frequency. Indian
Peafowl emits a wide variety of calls
comprising of single note calls, alarm calls
and breeding season calls. These calls
encompass a wide range of frequency and can
be categorized into short range (0-4kHz) and
broad range calls (0-15kHz). Calls were
associated with specific behaviour(s) and
the situations viz. the “Honk”, “Pe”,
“Honk-Kor-Ko-Ko” and “-Kor-Ko” was emitted
in the presence of threat, Whereas, “Pe-Kaan”,
“Honk-Kaan”, “Kor-kaan”, “Eow” and “Kaan”
was specifically associated with the
breeding season. Our data suggest that the
calls varied across the breeding and
non-breeding season and Indian Peafowl are
more actively vocal during breeding season
as compare to the non-breeding season. This
study will provide baseline data for further
research on Indian Peafowl vocalization. |
Masters Dissertations – 2018
|
Project title:
Identification and photo-documentation of
Myxobacteria from soils of Pune
Name of the student: Rama Paranjape |
|
Myxobacterial diversity
is one the rare area of microbial diversity
and at the same time wide area of interest
due to their characteristic features of life
cycle .i.e fruiting body formation.
Myxobacteria is Aerobic, Gram Negative, and
Saprophytic Organism which feeds on other
microbes for nutrition. They switch their
mode of life cycle from vegetative to
reproductive form depending upon nutrient
conditions. Wide range of social adaptions
and life cycle complexity involving co-
perative behaviour broaden the interest in
research field.
This study Revealed,
Two myxobacterial strains viz. Myxococcus
Xanthus and Un-indentified spp. were
isolated from diverse habitat including
urban and sub- urban areas using Rabbit dung
method and cultivated using water agar
medium. Morphological characterization
revealed vegetative short rods were
confirmed by biochemical analysis. Life
cycle stages such as swarming, aggregation,
Fruiting body formation, and Sporulation
were reflected as a result of photo-
ocumentation. Saprophytic predatory nature
of Myxobacterial species was recorded gave
indication of Antimicrobial Compound
formation by the cells as a secondary
metabolite. Antimicrobial susceptibility of
prey organisms including pathogens against
Myxobacterial cell extract was the measure
of bacteriolytic nature of Myxobacteria. |
|
Project title:
Evaluation of antimicrobial activity in
extracts from marine algae
Name of the student: Rutuja Tapkir |
|
Evolution of
Antimicrobial activity in extracts from
marine algae. In the last three decades the
discovery of metabolite and biological
activities from marine algae has been
increased significantly. Seaweeds known as
macroalgae are amongst the most important
primary producers and act as ecological
engineers on rocky coast of the world’s
oceans. Marine algae are known as a
potential source of bioactive substances.
Antimicrobial activity indicates that the
presence of active constituents in the
extraction of marine algae which can be
exploited for the production of innovative
drugs for the benefit of the humanity. In
this study it was shown that aqueous extract
of green algae Enteromorpha ramulosa has
ability to inhibit the growth of the gram
positive and gram negative bacteria. However
the ethanolic extract showed no
antibacterial activity. Antifungal activity
was not detected against Aspergillus niger.
Antioxidant activity by DPPH assay was
determined. For the purpose of phytochemical
investigation preliminary phytochemical test
and TLC were mainly used and which showed
presence of various phytochemicals. GC-MS
analysis of ethanol extract of Enteromorpha
ramulosa shows the presence of constituents
like
3-(4-Ethoxy-phenyl)-1-phenyl-benzo[f]quinoline,
Diisooctyl phthalate and Bis(2-ethylhexyl)
phthalate reported that having various
biological activity |
|
Project Title:
Importance of Urban woodlands as bird
habitats: A case study of Pune city region
(MS)
Name of the student: Fergus Anthony |
|
Biodiversity
conservation in urban areas has become
significant not only because of increasing
human population in urban centers but also
because it is one of the innovative ways to
conserve biodiversity as suggested by
various global environmental conventions.
This Study was carried out for the period
from July 2017 until February 2018 aimed at
comparing the bird communities between Urban
woodlands and a Natural forest situated at
the periphery of the city of Pune, grasping
the influence on the birds in the urban
woodlands, and ultimately suggesting methods
of preserving and managing the urban
woodlands in a proper manner so that the
biodiversity is conserved there. The number
of birds observed in this study recorded 83
species and 1103 individuals in total, where
the number of species were 68 in urban
woodlands and 47 in the natural forest. The
number of Individuals were calculated by
taking average values per visit as the
number of visits were unequal which resulted
in Natural forests having higher number of
Individuals observed there, this is mainly
due to the levels of disturbance which is
almost nonexistent in the Natural forests
but present in the urban woodlands.
I also compared the
possible biotic and abiotic pressures that
the habitats face by preparing an impact
matrix and scoring them values from 0-5
(where 0 is not persistent and 5 is for
highly persistent).According to the matrix
the major factor affecting the biodiversity
in the corresponding areas was Human
intervention, recreation & settlement around
the woodlands and a majority of the factors
are human induced which must be controlled
as early as possible. By this study we can
see that the urban woodlands of Pune city
have a high avifaunal richness and
preserving it is very essential. Since
increasing the size of parks is difficult in
the city, enhancement of habitat diversity
and resources availability for birds within
urban woodlands appears to be a
straightforward way of increasing urban bird
diversity. |
|
Project Title:
Foraging ecology of free ranging dogs in
Pune city
Name of the student: Malvika Colvin |
|
Free-ranging dogs, (Canis
lupus familiaris) are an integral part of
the human environment in India and many
other countries. They spend their entire
lives on the streets as scavengers, and
though they are not owned by humans, they
are dependent on humans for their
sustenance. In India, dogs are considered to
be ‘neighbourhood dogs', being either
semi-dependent or independent of people for
food and shelter and unrestricted in their
movements. This neighborhood dog's account
for about 60 percent of reported dog-bite
injuries.There is a need to manage and to
control free-ranging dogs as the dog’s bites
incidences are being still reported. As part
of an extended study on the Foraging ecology
of dogs in Pune, I tested the hypothesis
that active feeding in dogs will lead to
less foraging and difference in time budget
of urban stray dogs. If this hypothesis
holds, then the per capita effects of dogs
can be reduced by improving the quality and
quantity of their diet. For this study focal
animal observation method was used and
individuals were followed. Observations of
individuals were made from a vehicle or by
walking using an android application for
calculating the duration of activity. From
the observation, I used data to draw up a
frequency and time- activity budget of
community fed dogs and free-ranging dogs.
Further analysis was conducted to compare
the behavior of dogs which are fed and which
are not. Two major parameters were used for
comparison of foraging behaviors: Treatment
and Site. Our analysis showed a significant
difference in behavior of free- ranging dogs
and community fed dog with respect to active
feeding. This reveals that the community fed
dogs spend less time in foraging as compared
to free- ranging dogs. But in other
activities such as playing, social
interaction, barking, etc there is no
significant difference between community fed
and free-ranging dogs. This implies that,
despite feeding dogs, there is no major
change in their behavior. Further research
is required to determine if feeding results
in changes in local distribution and density
of dogs and if this has the potential to
exacerbate conflict. |
|
Project Title:
Effect of agricultural operations on soil
micro flora & its correlation to
bio-fertility.
Name of the student: Shantanu Ekbote |
|
The agricultural soil
is subjected to various operations and
practices in the course of its use for crop
production. The soil health is affected due
to the operations like tilling, ploughing,
weeding out grasses and use of weedicide.
This affects the already critical stage of
organic carbon content of soil. The
rhizospheric beneficial oligotrophic
microflora associated with soil and plants
also gets affected by such disturbances.
Hence the area of focus was oligotrophs. In
this study, a farmland with no operations
except tilling & addition of nitrogen was
compared with farmlands implying the above
mentioned practices. The diversity
pre-enrichment and post-enrichment was
represented on 8 different media specially
designed for oligotrophs. At numerous
instances in statistical analysis of data,
the diversity and evenness were found to
have relatively higher values for the
former. The latter was found to contain
nutrients in excess which could have been
the cause for lower counts of evenness and
diversity indices. The nutrients included
NPK, micronutrients & physico-chemical
parameters. The oligotrophic microbial
diversity was affected due to certain
practices implied at that instant. The
co-relations obtained from study were
subjected to co-relation with physico-chemical
parameters of the soil. |
|
Project Title: To
investigate diversity of Rodents (Muridae)
and their prey-predator relationship with
Canidae (Canis lupus, Vulpes bengalensis) in
Semi-Arid Grassland ecosystem, Solapur,
Maharashtra.
Name of the student: Akshay Waghmare |
|
The attempt was made to
understand the ecology of the habitat by
studying some crucial aspects of ecology
like Diversity, and prey-predator
relationship. Diversity of rodents was
studied for the first time in Solapur.
Understanding the diversity of rodents can
also give clues for studying availability of
prey species for the carnivores. The
availability of prey species can vary with
the habitat and vulnerability. Anthropogenic
pressure can lead to decline in the
population of the major prey species and
hence affecting the predators. This can also
lead to change in the feeding habits. As
wolves and foxes are well known for their
diversified feed it is important factor to
study this aspect on temporal and spatial
scale. Rodents being the major part of fox
diet it is necessary to study and understand
the prey selection so as to know the niche
preference and prey availability. Habitat
utilization by these carnivores can also be
known along with this study as some rodents
require specific habitats throughout their
life history. |
|
Project Title:
Diversity of insect pollinators in Amboli
Name of the student: Jitendra Marathe |
|
The attempt was made to
understand the ecology of that habitat by
studying some crucial aspects of ecology
like Diversity, and prey-predator
relationship. Diversity of rodents was
studied for the first time in Solapur.
Understanding the diversity of rodents can
also give clues for studying availability of
prey species for the carnivores. The
availability of prey species can vary with
the habitat and vulnerability. Anthropogenic
pressure can lead to decline in the
population of the major prey species and
hence affecting the predators. This can also
lead to change in the feeding habits. As
wolves and foxes are well known for their
diversified feed it is important factor to
study this aspect on temporal and spatial
scale. Rodents being the major part of fox
diet it is necessary to study and understand
the prey selection so as to know the niche
preference and prey availability. Habitat
utilization by these carnivores can also be
known along with this study as some rodents
require specific habitats throughout their
life history. |
|
Project Title:
Chemical and biological examination of Musa
(banana) leaves: A special reference to leaf
position
Name of the student: Neha Tone |
|
Plants produce large
number of metabolites (phytochemicals) that
play important role in growth, development
and response towards environment. It is well
known that a wide range of chemical signals
enable them to optimize their adaptation to
their respective environments, termed as
defensive chemicals or allelochemicals.
Through this study, we aim to understand the
influence of leaf position on the production
of metabolites. One such study was performed
on Musa (banana) species. Musa is a
perennial herbaceous crop easy to obtain and
work with. In this work we investigate the
influence of leaf position on phytochemical
profile. The leaves selected for the study
were categorized as foliage, middle and the
cigar leaf. They were collected from three
different Musa plants and were screened
using ethanol: chloroform: hexane extracts
for comprehensive assessment of the
biological activities. The variations in the
presence of the phytochemicals may also be
due to the choice of the solvent used in the
extraction, ethanol is a polar solvent while
hexane is a non- polar. Variation in
phytochemicals and biological activities
were observed in different developmental
positions, thus contributes in finding
characteristic metabolites (metabolic
markers) for specific developmental stages.
Phytochemical study revealed the ethanol and
hexane extract showed high antimicrobial
activity as compared to chloroform.
Antioxidant study revealed no correlation
with the position of leaf and collection day
but a positive correlation in the tine of
exposure to the reducing potential was
observed. This study may provide unique
insights into understanding the fundamental
nature of plant phenotypes in relation to
development, physiology, resistance and
biodiversity. These results may aid plant
breeders to identify resistant marker
metabolites that integrate phytochemical
data with the influence of the environment
under stress conditions. |
|
Project Title:
Evaluation of Phytotoxic effects of leaf
leachates of Cosmos and Xanthium on crops
such as Triticum aestivum L, Trigonella
foenum-graceum L, Vigna radiata L.
Name of the student: Pallavi Gharpure |
|
Many invasive weeds are
reported world-wide. Studies pertaining to
them suggested their deleterious effects on
biological ecosystems. Deleterious effects
such as inhibition of growth of indigeneous
plants and agricultural crops, bringing
about drastic changes in soil characters.
Weeds such as Cosmos and Xanthium have their
existence near agricultural crops field.
Such weeds differ in abundance, which
release specific allelochemicals or
ecochemicals which have adverse effect on
germination rate and their physiological
patterns and reproduction. Allelopathic
effects of leaf leachates of Cosmos and
Xanthium were studied on seed germination
and seedling growth of Triticum aestivum
(Wheat), Vigna radiata ( Mungbean) and
Trigonella foenum-graceum (Fenugreek )
mainly cultivated crops from Pune region.
Seed germination was inhibited at higher
concentration at 6% while lower
concentrations showed some stimulatory
effect on Mungbean and Fenugreek from 1%-4%
concentrations. But seed germination
percentage of Vigna and Trigonella
foenum-graceum showed 70% and 60% growth in
response to leaf leachates concentration of
Cosmos at 6%. Triticum showed total
inhibition of 40% to Xanthium and Cosmos
leaf leachates. The qualitative analysis of
phytochemicals showed presence of alkaloids,
phytosterols, phenols, tannins, flavonoids
with absence of saponins, carbohydrates and
proteins. Variations in results can be
concluded due to different environmental
condition response of the plant. GC-MS and
IR studies revealed presence of major
constituents as esters, ethers, anhydride
and polyalcohols. FTIR analysis of Cosmos
and Xanthium in the range 4000-400 cm-1
showed the characteristic fingerprinting
regions of various functional groups such as
–OH, carbonyl, anhydride, ester, amide. |
|
Project title:
Development of attractant and repellent
formulations for pollinating Butterflies
Name of the student: Preet Deoghare |
|
The project is based on
the idea to increase the rate of pollination
by using butterflies as a tool. Many plants
attract and reward pollinators with floral
scents and nectar. Attracting butterflies by
using attractants when they supposed to
pollinate and repelling them or keeping them
away from the flowers when fertilizers have
been spread on the crop plants is the basic
principle behind the project. For attractant
and repellant formulations, essential oils
of flowers of Lantana camara, Conoclinium
coelestinum and Cosmos sulphureus were used
at various concentrations. The study is to
reveal the ecological interaction between
butterflies and flowers using various
concentration of essential oil at which they
respond. It is an ecofriendly way to
increase the rate of pollination with the
help of biotic factors and ecological
functions being performed in the nature. The
bioactivity of essential oils of these three
species was checked over four concentrations
(1, 5, 25 and 50 μl) with liquid paraffin as
a solvent. Six replicates of each
concentration were taken. Visual cues (Red
and Yellow) colours were tried along with
olfactory cues. We got no significant visits
of butterflies on the tried plant species
and their various concentrations. |
|
Project title:
Characterization and Identification of
Actinomycetes and their bioactive potentials
Name of the student: Sayed Irfan |
|
Actinomycetes are
beneficial soil bacteria and potential
candidates as biocontrol agents where most
of the antibiotics are produced by these
microorganisms. The use of such microbes
against other disease-causing bacteria is an
attractive and ecological approach. The
study reports fermentative production of
bioactive compounds from Streptomyces
cultures which were checked for their
antimicrobial activity. 13 cultures out of
58 cultures showed activity against bacteria
which was then chosen to check their Minimum
Inhibitory Concentration. Selected cultures
showed good activity against bacteria and
yeast such as Staphylococcus aureus,
Staphylococcus epidermidis, Pseudomonas
aeruginosa, Escherichia coli and Candida
albicans, respectively. KG3 strain (Streptomyces
luteosporeus) showed good activity against
all strains and its MIC was very less
against E.coli that is 2.5μg / ml. V18
strain (Streptomyces hydrogenans) MIC
against C.albicans was 182 μg / ml. These
selected cultures were then characterized
phenotypically by staining, SEM imaging and
growing on different media such as AIA, SCA,
MGYP, ISP-2, and ISP-7. Biochemical
characterization and 16SrRNA approach was
used to identify the potential strain.
Laboratory Scale Fermentation of one isolate
i.e. KG3 was carried out. Separation of the
bioactive compounds produced by the selected
strains was carried out using TLC
bioautography and High Performance Liquid
Chromatography profiling of the same was
carried out. Based on sequence data
phylogenetic relationship of the potential
strain was established. |
|
Project title:
Diversity of insect induced plant galls in
Amboli
Name of the student: Shantanu Ghatge |
|
Tumors caused on
vegetal part of the plants, named galls
occur due actions of various organisms such
as bacteria, fungi, nematodes, insects and
mites. The galls induced by insects show the
most complex type of herbivory. Most of the
gall-inducing insects show high specificity
towards host plant. A survey was carried out
from August 2017 to February 2018 in Amboli
to study the diversity of plant galls,
morphological characters of plant galls and
host plant alteration by gall inducing
insects. During the survey, sixteen
morphotypes of galls belonging to 12 plant
families reported. Families Myrtaceae,
Melastomaceae and Lauraceae presented the
highest number of galls. Gall inductors
belonged to orders Diptera, Hemiptera,
Thysanoptera and Lepidoptera. Most preferred
organ for induction of gall was leaf. Data
on the parasitoids of gall inductors was
also incorporated in the present study. |
|
Project Title:
Taxonomic identification of Hermit crabs and
their shell utilization pattern from Kelshi,
Maharashtra; India
Name of the student: Shraddha Athavale |
|
The purpose of present
study was to investigate the taxonomic
identification and the shell utilization
pattern of the hermit crabs found on Kelshi
beach. The samples were collected from
November 2017 to January 2018. Sampling was
carried out on two sites: rocky patch and
sandy beach. At both the sites to get
maximum diversity of hermit crabs,
stratified sampling was done at high, mid
and low tide areas. Total 5 species were
identified after examining the taxonomic
characters. Color pattern, chelipeds, telson,
rostrum and parts of ambulatory legs were
found to be important in taxonomic
separation. Clibanarius arethusa and P.
kulkarni were found using only 3 and 5
species of gastropod shells respectively
during the study period. While C. padavensis
was using 10 shell species, C. zebra was in
15 shell species, D. dubius was found in 15
gastropod species. It has been observed that
ovigerous females use only specific types of
shells. The difference between shell
utilization by males and females of single
species was also observed. For conclusive
remarks on the shell preference by each of
these species of hermit crabs, more samples
needs to be collected. |
|
Project title: In
vitro studies on allelopathy by the effect
of leachate on germination of crop seeds.
Name of the student: Tasmiya Sheikh |
|
The phenomenon of
direct or indirect influence of one plant on
the other plant , animal or microbes is
known as allelo biogenesis. It occurs
through the variety of allelo chemicals or
eco chemicals produced by the plant. These
interactions are positive as well as
negative. The phyto chemicals produced by
the plants are naturally released in the
soil in the form of leachates. Naturally
these leachates are found to be affecting
seed germination process of other plants. So
it was conceptualised that whether these
leachates are showing their effect on seed
germination under in vitro conditions. So
the weed plants are selected to study their
allelo biogenetic effects on seeds of some
crop plants in invitro conditions. Cosmos
sulphureus and Xanthium strumarium plants
were used in plant tissue culture. Different
concentration of leachate was used (
40%,20%,10%,5%,2.5% )to know the growth of
plant in invitro. By leachate it was seen
that it was inhibiting the growth of crop
seed plants. Phytochemical analysis was done
of Cosmos sulphureus and Xanthium strumarium
many chemicals showed positive results. |
Masters Dissertations – 2017
|
Project Title:
Effect of hypergravity on survival and
antibiotic resistance of bacteria
Name of the student: Akshay Marathe |
|
Ecology The distinctive
feature of brown dwarfs, sub-stellar bodies
and Jupiter is the presence of hypergravity.
The fundamental requisite for survival in
such environment is the ability to
demonstrate growth in hypergravity. Besides
survival in hypergravity and rocks, it is a
mandatory requirement for microbial travel
between planets, panspermia, and resistance
to hyper accelerative forces faced during
ejection of rocks from planet. As survival
of organismic life in marine and
hypergravity is mandatory for substantiating
theories of panspermia and inter planetary
travel, this study aimed at exposure of
Escherichia coli KK1 and Vibrio spp KK2 to
hypergravity. Escherichia coli KK1 and
Vibrio spp KK2 previously isolated in our
laboratory from marine sample were subjected
to hypergravity at 893 X g using
acceleration generated by centrifugal
rotation. The cells of Escherichia coli KK1
and Vibrio spp KK2 proliferated and
demonstrated good growth in hypergravity
(893 X g). Both were also tested for various
modes of action for combating hypergravity.
Various effects of hypergravity(effect on
membrane permeability, antibiotic
susceptibility etc.) were determined. The
current investigation highlights that
hypergravity may not be a limitation for
habitability of sub stellar bodies or
planets with hypergravity. |
|
Project Title:
Ecofriendly Approach to Enhance Soil
Fertility for Plant Growth, Yield and Status
of Crop
Name of the student: Naik Akshada Yashwant |
|
Spinach is commonly
found, high demand widely grown leafy
vegetable in India, especially in the state
of Maharashtra. The fresh leaves of Spinach
are a rich and cheap source of vitamin A,
vitamin C, iron, essential amino acids,
Ascorbic acid etc. Considering the weather
conditions Spinach is sowed in this region
at two times of the year viz as a main crop
in October and further in January- February
as a summer crop. The plant grows to a
height of approx. 30 cm. The growth of the
spinach plant is stimulated and promoted by
many components mostly present naturally
which can also be enhanced through
artificial means such as plant growth
promoting and regulating microbes. Said
microbes when inoculated with biogas slurry
have shown more potency and thus can compete
with other available microbial flora. This
is further supplemented through use of
present day nanotechnology techniques to
provide quick results.
This project focused on
consortium of these three approaches to
study growth of Spinach plant namely
Biofertilizers, Nanoparticles and biogas
slurry. It studied how these combinations
can be useful in stimulation of plant
growth, yield and status. Nanoparticles were
characterized through U.V. spectrometry, SEM,
FTIR and XRD. Concentration of silver and
zinc nanoparticles (1ppm to 10ppm) did not
show antagonistic effect against Phosphate
solubilising microbes and Bacillus subtilis
microbes. Also, they did not show any toxic
effect which was confirmed using Allium cepa
assay.
The results showed that
when tested for growth and yield of a
spinach plant (field trials) by applying
biofertilizers combination along with
different nanoparticles, silver nanoparticle
had higher potency followed by zinc
nanoparticles. Thus, silver nanoparticles
consortium can act as a promoting future
nanonutrient. |
|
Project Title: A
Study of Odonate Species Assemblage across
an Urbanization Gradient
Name of the student: Apeksha Darshetkar |
|
Understanding species
responses to habitat modification and
urbanization are important to understanding
their specific conservation needs. Odonates
(Dragonflies and damselflies) are freshwater
insects looked upon as good ecological
indicators for understanding wetland habitat
health. To understand responses of tropical
odonates to urbanization gradient, we
carried out a study on habitat ecology of
odonates along an urbanization gradient at
six sites along Mula River that flows across
Pune City. As a part of the standardization,
we first evaluated four counting methods for
sampling adult odonates at river-side –
Full-width Belt Transect, Half-width Belt
Transect, Full-circle Point Count, and
Half-circle Point Count. We then recorded
species and their habitat correlates by
taking several temporal replicates. We used
one-way ANOVA to compare among sampling
methods. For species-habitat analysis, we
first performed variable reduction using
Principal Component Analysis (PCA) and then
performed Redundancy Analysis (RDA) and
Canonical Correspondence Analysis (CCA) on
species-habitat data. We found that
Half-circle Point Count method was the best
suitable method to count adult odonates at
river-side. We documented 35 odonates across
six sites. Our multivariate analyses did not
return statistically significant results;
however, damselflies showed a shift in the
species composition across the urbanization
gradient as compared to dragonflies. Here we
show that traditional belt transect method
is not suitable for counting adult odonates
at river-side and species responses to
urbanization may vary based on their habits
and ecology. We further discuss the
implications of the result and where future
researchers should focus. |
|
Project Title:
Spatial and temporal phytoplankton
biodiversity dynamics of Mutha River, Pune
Name of the student: Aseem Abhay Shendye |
|
The attempt was made to
understand the ecology of the habitat by
studying some crucial aspects of ecology
like Diversity, and prey-predator
relationship. Diversity of rodents was
studied for the first time in Solapur.
Understanding the diversity of rodents can
also give clues for studying availability of
prey species for the carnivores. The
availability of prey species can vary with
the habitat and vulnerability. Anthropogenic
pressure can lead to decline in the
population of the major prey species and
hence affecting the predators. This can also
lead to change in the feeding habits. As
wolves and foxes are well known for their
diversified feed it is important factor to
study this aspect on temporal and spatial
scale. Rodents being the major part of fox
diet it is necessary to study and understand
the prey selection so as to know the niche
preference and prey availability. Habitat
utilization by these carnivores can also be
known along with this study as some rodents
require specific habitats throughout their
life history. |
|
Project Title:
Assessing abiotic ecological parameters of
sand influencing direction of root growth of
Ipomoea pes-caprae (l.) on sandy beaches of
northern Ratnagiri, Maharashtra
Name of the Student: Chinmaya Ghanekar |
|
The species Ipomoea
pes-caprae is perennial, creeping vine
having bilobed leaves and violet
infundibular flowers. Roots of I. pes-caprae
commonly grow in vertical direction i.e.
perpendicular to sand surface. Reportedly,
roots of I. pes-caprae are also found to
grow lateral i.e. parallel to sand surface.
Also these roots are known to threat Olive
Ridley Turtle nestings from Maharashtra.
Considering this, variation in direction of
root growth became important to study in the
light of abiotic ecological factors such as
temperature, moisture, organic carbon and
sand grain size. This study established a
strong correlation between fine grain
proportion in sand with vertical root growth
and medium grain proportion in sand with
lateral root growth. Therefore, it can be
inferred that mechanical resistance provided
by different sizes of sand grains may have
effect on direction of root growth of
Ipomoea pes-caprae. This study also
discusses about refining conservation
practices for Olive Ridley Turtle nestings.
Along with the lab based analysis, this
study attempts to monitor root growth of the
species Ipomoea pes-caprae by a small
experimental setup where original growth was
removed and a new growth was observed in
relation with the sand grain size for which
the qualitative results are provided. |
|
Project Title:
Habitat preference of Bamboo Pit Viper (Trimeresurus
gramineus)
Name of the students: Devendra Dutta Pandey |
|
I studied factors
influencing habitat selection by T.
gramineus (Bamboo pit viper). To determine
microhabitat selection, a range of habitat
features at each snake’s location were
measured. Whether or not the animal was
found in a tree, its height of perch,
position on the branch (distal/
apical/middle), diameter of the branch, the
tree canopy (thick/sparse) and vegetation of
the area (thick/sparse) were recorded.
Assessment of habitat was done to determine
how patterns of habitat use vary seasonally.
Shaded ambient (air) temperatures and
humidity were recorded. No individual was
found in open habitats. The individuals
preferred diverse habitats and were spread
over the entire available space during the
monsoon; bamboo pit viper did not show any
preference for the perch height during
different seasons. The present study
suggests that several factors play an
important role in habitat selection by these
arboreal pit vipers, thus making them highly
habitat-specific. |
|
Project Title:
Understanding the response of people living
in Protected Area (Sanjay Gandhi National
Park, Mumbai) towards leopards.
Name of the Students: Nishant Nandkumar
Zajam |
|
In megacity like Mumbai
where land is a scarce source, Sanjay Gandhi
National Park is extremely important for the
conservation of Leopard Panthera pardus
fusca. SGNP has been surrounded by the sea
of human settlements across its periphery,
causing habitat degradation of the apex
predator of the park. Human- leopard
interactions occur frequently in SGNP, as
the leopard enters into the human habitation
inside and outside the park in the search of
the prey. The tribal community living inside
SGNP show positive response of the presence
of leopards in their vicinity. Documentation
of the response and views shown by the
tribal community towards the leopards is
essential in understanding the approaches of
tribes towards the large predator. This
information can be used for planning long
term leopard conservation project in SGNP.
This study deals with the different response
shown by the tribal community in SGNP
towards leopards. |
|
Project Title:
CHARACTERIZATION OF SEED AND FRUIT TRAITS OF
WILD WOODY PLANT SPECIES FROM AMBOLI, DIST.
SINDHUDURG, NORTHERN WESTERN GHATS OF
MAHARASHTRA
Name of the student: Pooja Ghate |
|
Seed is a product of
sexual reproduction that consists of an
embryo and seed coat. It disperses via a
vast range of mechanisms, involving both
abiotic and biotic vectors. It plays
critical role in species establishment and
growth in the given habitat. Very few
ecological studies concerned with seed
traits have been conducted for tropical
woody plant species in general and Western
Ghats (WG) in particular. Considering
importance of seeds in plant diversity
establishment, study on seed and fruit
traits of woody plant species from Amboli in
Northern Western Ghats was initiated. Of the
total 44 species characterized for seed and
fruit traits, 7 species were endemic to WG
and 4 belong to ICUN red category. Data on
seed and fruit traits such as fruit mass,
fruit dimensions, fruit colour, fruit type,
number of seeds per fruit, pulp to seed
ratio, seed mass, dimensions, seed colour
and shape were recorded. Relation of these
traits with plant attributes like evergreen
and deciduous nature, canopy and understory
strata, height of trees and dispersal mode
and season of dispersal was investigated. Of
the total number of species studied, about
50% species (21 species) were single seeded
and all exhibited zoochorous mode of
dispersal. ‘Black’ was found to be the most
common colour of mature fruits followed by
‘red’ among the studied species. The most
dominant fruit type was berry (48 %).
Dysoxylum binectariferum has the highest
fruit mass (48g). Presence of under
developed, immature seeds was more common in
case of multi seeded fruits as against
single seeded species. 48% species showed
seed mass within 0.1-1.0 g. Highest pulp to
seed ratio was observed in Salacia chinensis
(9.98) whereas Beilschmiedia dalzellii has
lowest ratio (1.5). Maximum number of
species (20) were found to be dispersed in
summer season (45%) followed by rainy season
17 (39%), followed by winter season 7 (16%).
Weak relation (r =0.39) was found between
the seed mass and the height of the tree. |
|
Project title: To
study Abundance, Occurrence and Seasonal
patterns of aquatic Beetles of Sinhagad
fort, Pune
Name of the Student: Pragati Shinde |
|
Coleoptera is one of
the species rich group of Insecta. An
attempt was made to study seasonal patterns
of aquatic beetles on Sinhagad fort from
June 2016 to March 2017. Thirty three
species belonging to four different families
of aquatic beetles were observed. Out of 33
species, 7 were common, 7 were average and
19 were rare species. Laccophilinae and
hydroporinae show highest co-occurrence. Mud
based water bodies and rock based water
bodies show two different clusters (Jaccard
index 0.2). Temperature and pH vary between
season while salinity and number of species
did not vary. The ecological variables as
area, vegetation and salinity show
significant effect on the occurrence of
aquatic beetle species. |
|
Project Title:
Taxonomy, distribution and threats to
Shield-tailed snakes (Reptilia: Serpentes:
Uropeltidae) in Satara District, Maharashtra.
Name of the Student: Prasad Gopalkrishna
Gond |
|
Uropeltidae is a family
of cyanophidian snakes with regard to
taxonomy, distribution, and threats are one
of the least studied and lesser documented
family of snake from India and Sri Lanka.
Broad range distribution of Uropeltidae
family is known from Maharashtra. The study
aimed at mapping the distribution at micro-
cale level by using various indirect
sources. In the present thesis, I studied
literature review and museum specimens.
Occurrence of road killed individuals were
used to analyze species richness among
Uropeltidae family in Satara district of
Maharashtra state. Based on this information
further study on ecology and behavior of
these species from this region can be done.
This research provides a simple model using
secondary data to map the distribution of
other lesser documented family of snakes. |
|
Project Title: To
check the diversity of short horned
grasshoppers in various habitats of selected
localities of Pune: Mulshi, Sinhgad valley,
Nanded phata, ARAI hill.
Name of the Student: Priyanka Ram Bansode |
|
Short horned
grasshopper diversity was checked in various
habitats of selected localities from Pune.
Orthoptera is one of the largest orders of
grassland insects. Orthopterans are
distributed through the physiographic zones
of the world but their distribution largely
depends upon the vegetation like grasslands,
forests and agricultural fields. (Gangwere
et al., 1997). Grasshoppers are of great
economic importance, because they constitute
an important group of pests and pose a
constant threat to cereal crops, pulses,
vegetables, orchards, and grassland and
forest plantations all over the world.
Grasshoppers cause significant damage to
tree seedlings and agricultural crops (Joshi
et al., 1999). Sweep netting and direct
sampling with active search methodology was
used for catching the grasshoppers for
identification purpose . Grasshoppers were
killed , pinned and identified with help of
previous key and literature data. Total 704
individual of 2 families 11 subfamilies , 14
genera and 17 species was encountered during
the study . Trilophida annulata Thunberg was
relatively the dominant of all species in
all habitat and localities . Result shows
that there does not seem to be any effect of
month or habitat type on abundance of
species But the diversity indices for
Simpson 1D , Shannon H is relatively high
for grassland as compared to agricultural
land and forest land. |
|
Project Title:
Influence of decreasing surface tension on
water-strider locomotion, with assessment of
seta morphology and allometry of locomotary
legs
Name of the Student: Renuka Kulkarni |
|
Water-strider
locomotion has long been studied for its
propulsive fluid dynamics on the surface of
water, and the hydrophobicity rendered by
the microstructures present on the insects’
water-repellent hair. While it is a
well-established fact that the high surface
tension of the water surface helps these
insects walk on water, no studies had
attempted to explore how water-striders
respond to decreasing surface tension. This
study conducted experimental trials where
two local species of Gerridae, Limnogonus
fossarum and Tenagogonus fluviorum, were
subjected to decreasing levels of surface
tension, and found that neither species
could remain afloat beyond a surface tension
of 0.035 Newton/m. L. fossarum showed a
higher sensitivity in response to such
changing conditions. This study also
examined seta microstructure of seven local
species of Gerridae, L. fossarum, T.
fluviorum, Aquarius adelaidis, Onychotrechus
rhexenor, Cylindrostethus productus,
Metrocoris indicus and Ptilomera agriodes
using Scanning Electron Microscopy, and
found previously undocumented differences in
types of seta as well as in the arrangement
of nano-grooves on the seta. Multivariate
allometry of the mid and hind legs of two
species was also performed to understand
variation in the length of these two
locomotary appendages. Allometric analyses
revealed an overall similarity in the mid
and hind-leg length, except for a remarkable
variation in L. fossarum with respect to the
length of the second tarsal segment of the
mid-leg. This may be a case of sexual
dimorphism or a character unique to the
species. Further studies needed to explore
this variation in these and other Oriental
species of aquatic Hemiptera. |
|
Project
title: Composition and changes of Araneae
fauna, Spider (Arthropoda: Aracnida) in the
agriculture field in relation with growing
crops.
Name of the student: Rishikesh Tripathi |
|
The study tested the
hypothesis that effect of crop growth on
spider population and composition of spiders
changes with respect to physical structure
of crops. To test the assumption two
structurally different crops were selected,
Bajra-Pearl millet (Pennisetum americanum)
this is standing crop and Groundnut (Arachis
hypogaea) which is spreading crop. Sampling
was done for one complete season from sowing
to harvesting. It was noted that with every
succeeding visit spider population was
increasing. Also, it was observed that
web-building spiders were directly linked to
the configuration of the vegetation because
of specific web attachment requirement.
Both, observation and analytical data
support a strong relation between spider
density and habitat structure. More
investigation into the specific of how
habitat structure influences the
prey-predator interactions in agrosystem is
needed in order to truly understand and
manage agriculture production in a
responsible manner. |
|
Project title
How has Tamhini Ghat Changed? Comparing
Butterfly populations in a threatened
habitat.
Name of the student: Shawn Dsouza |
|
Tamhini –
Sudhagad Wildlife sanctuary
(18o27’N,73o25’E), established in 2013, is a
prime example of crucial habitat giving way
to human encroachment. Voluminous research
has established butterflies to be sensitive
to and hence indicative of ecological
disturbance. This study intended to
highlight the patterns of change in
butterfly populations from 1998 to 2017,
amidst changing land use in the Northern
Western Ghats biodiversity hotspot.
Butterfly diversity & evenness, community
structure and community composition were
compared across seasons (temporal), habitats
(spatial) and six dietary guilds based on
host plant preference, viz., grass
specialist, herb specialist, liana
specialists, shrub specialist, tree
specialist and generalist. These patterns
were compared to data published by Padhye et
al. (2006) collected between 1998 and 2000.
There is a significant increase in diversity
over two decades (𝐻𝐶𝑢𝑟𝑟𝑒𝑛𝑡1 = 28.31,
𝐻𝑃𝑟 𝑣𝑖𝑜𝑢𝑠1 = 22.31, p << 0.01).
However, unevenly distributed abundance
along with an increase in generalist species
are indicative of disturbance and higher
habitat homogeneity. High diversity values
coupled with the high species turnover (Bray
– Curtis dissimilarity = 0.3283) may be
caused by a higher number of satellite
species foraging in the study area.
Community structure of butterflies resembles
communities in early stages of succession.
The changes in the community over the period
of almost two decades are indicative of
increasing levels of anthropogenic pressure.
Long-term continuous monitoring could
provide deeper insights into the effects of
unchecked development and habitat
modification has on threatened ecosystems. |
|
Project title:
Spatial and temporal zooplankton
biodiversity dynamics of Mutha River, Pune.
Name of the student: Siddarth Sudhakar |
|
It has been
demonstrated that qualitative and
quantitative characteristics of zooplankton
depend on the degree of pollution of a water
body and adequately reflect differences in
the conditions of zooplankton growth between
the whole water bodies and their individual
parts. Cladocerans from the polluted river
Mutha, Pune, Maharashtra were sampled from
December 2016 to March 2017. pH, DO, BOD,
COD, TDS and Hardness was assessed from
water samples collected simultaneously, to
study such effects. This study describes
temporal and spatial changes in zooplankton
density and diversity, and the
physicochemical complex of the River. During
the study period individuals from 3 genera
belonging to the order Cladocera were
identified viz. Moina, Kurzia and
Ceriodaphnia. A sharp increase in the
cladoceran population was observed in the
month of March. Spatial differences in
zooplankton population were more apparent in
the winter months of the study period. The
spatial and temporal differences are
attributed to the combined effects of
various hydrological, physicochemical
factors across the river, effluent discharge
into the river and also the human activity
on the banks. |
|
Project title:
Assessment of diversity and abundance of
Birds, Butterflies, Odonates and Amphibians
in an artificially developed landscape and
natural forests
Name of the student: Srushti Bhave |
|
In the current study
Bird, Butterfly, Odonate and Amphibian
diversity of a resort “The Empower Activity
Camp” was evaluated. The campsite was
constructed by landscaping natural forest
which is still present around the Campsite.
The Campsite now has a number of fruit
plantations, and some native plants along
with some artificial water ponds. Total
number of species recorded from the study
were, 121 species of Birds, 44 species of
Butterflies, 21 species of Odonates and 10
species of Amphibians. The Sorensen
Similarity index revealed 0.66, 0.60, 0.69
and 0.89 similarities of Birds, Butterflies,
Odonates and Amphibians respectively between
the campsite and its neighbouring forest.
Amphibians were found to be using the
artificial pools to for breeding. Worldwide,
efforts are being made to integrate
biodiversity into building of resorts and
hotels. Our study shows that retaining
patches of natural habitats while
landscaping and using native plants for the
same can help sustain a healthy diversity.
However, more such studies will be required
to come up with a set of principles and
policies that can be used for future
developmental projects. |
|
Project title:
Impact of Relocation program on Livelihood
of the people living in Achanakmar Tiger
Reserve, Bilaspur, Chhattisgarh
Name of the student: Sunny Milind Thatte |
|
Relocation programs are
implemented for the protection of natural
resources, tiger habitats and maintaining
‘Inviolate Areas’ for wilderness. This in
turn affects the management of the Tiger
Reserve, especially the Critical Tiger
Habitat which in the case of Achanakmar
Tiger Reserve is synonymous with its Core
Zone. The Core Zone of the Tiger Reserve is
termed as a sanctum sanctorum. It is
supposed to have the least disturbance and
is maintained as an ‘Inviolate Area’. In
such areas it is concluded that co-existence
of humans and wildlife is not possible. If
any habitation or a village is locate in the
core zone of the tiger reserve where
coexistence is not possible the management
has to take recourse to voluntary
relocation. Such relocation is a complex
process and affects both wilderness and
humans in various ways. This study has been
undertaken in the Achanakmar Tiger Reserve
from where 6 villages were relocated. This
served as a good study area to assess the
impacts of relocation. Data collection on
various socioeconomic and ecological aspects
was collected from the sampled inhabitants
of these villages through administration of
questionnaires, Focus Group Discussions and
Informant Interviews. The data was analysed
using various statistical methods in vogue.
After analysing various variables, it was
concluded that the relocation process
conducted by the management of Achanakmar
Tiger Reserve has been a successful one, in
terms of all the variables considered. The
conclusion drawn through this study is that
if carefully done and monitored the complete
process of relocation may be handled
properly without hurting the sentiments and
putting the local communities to
inconvenience and further achieving the
conservation objectives as well, to maintain
‘ Inviolate Areas ‘ for conservation of
wildlife. A way forward has been suggested
to guide the process of relocation in
future. |
|
Project title: Study
of Plant Diversity Along Banks of River
Mutha, Pune,Maharashtra, India
Name of the student: Smt.Uma Ashok Kalamkar |
|
The rivers are
important channels of material and energy,
which are constantly reeling under the
impacts of human influences that often lead
to deterioration of the ecosystems and
reduction in biodiversity. The Mutha River
in Pune is an east-flowing perennial river
holding a lot of ecological importance. A
great amount of biodiversity has been
observed and recorded by experts along the
Mutha river. Pune city, known as “Oxford of
the East”, has been growing rapidly in both,
human population and new land acquisition
along Mutha river, thereby causing impacts
on the riparian vegetation. Hence this study
was carried out to document the plant
diversity in 9 selected locations along
Mutha river banks. Total 243 plant species
belonging to 204 genera and 69 families were
recorded from the study area, dominated by
herbaceous plants followed by trees and
shrubs. Maximum number of species was
recorded from Vitthalwadi followed by
Khadakwasla and diversity was found
decreasing downstream from Vitthalwadi. With
respect to micro-habitats, dryland habitat
dominated the study region, followed by
moist soil habitat, rocky habitat, marshy
and aquatic habitats. Most dominant families
in the study region were Asteraceae and
Poaceae, followed by Fabaceae, Malvaceae,
Convolvulaceae, Euphorbiaceae, Labiatae,
Mimosaceae and Moraceae. Cluster analysis of
study sites based on similarity in the
floristic composition revealed that the two
sites viz. Khadakwasla and Sambhaji Udyan
Backside were distantly related to each
other as well as to other sites. Nanded City
site is also showing dissimilarity with
other sites. Specific species found here
viz. Combretum ovalifolium Roxb and
Glycosmis pentaphylla (Retz) DC indicate the
presence of evergreen forest along Mutha
river in the past. Comparison with previous
studies reveals appearance of 59 new species
and disappearance of families like Naidaceae,
Molluginaceae, Campanulaceae, Getinaceae,
Nyctaginaceae, Chenopodiaceae, Orchidaceae
and Amaryllidaceae which were recorded
earlier. Garbage dumping, loss of habitats
especially shrinkage in marshy area,
disconnectivity in existing habitats,
pollution and establishment of invasive
species are the major threats to Mutha river
vegetation. |
|
Project title: To
find temperature preference in Garden lizard
(Calotes versicolor)
Name of the student: Vaidehi Yashwant Lele. |
|
The study was conducted
to estimate temperature preference (Tp) by
ectotherms like lizards, to observe the
response to temperature change on lizards. A
garden lizard (Calotes versicolor) is least
concern lizard species in IUCN list. In this
study we have recorded body temperature (Tb)
of Garden lizards (Calotes versicolor) with
T type of thermocouple probe. Also recorded
their microhabitat temperature with HOBO
data logger. Samples were collected from
Pune and Mahableshwar study sites. These
sites were selected because altitudinal
difference. Total 10 individuals collected
from each site & noose sampling method was
used for sample collection. For this total
20 individuals we have recorded data of
location, altitude, SVL (snout-vent length),
sex, average Tb. This study we observed Tp
range for Pune sample was more than
Mahableshwar sample. Also SVL and altitude
change affect average Tb of lizards. We also
observed males are larger in size as
compared to females. |
|
Project title:
Microhabitat preference and locality records
of Sand Boas (Reptilia: Serpentes:Boidae)
Name of the student: Vishal. A. Varma |
|
The family Boidae
family is amongst the least studied family
of snakes in India. Three species are known
to occur in India out which Whitaker boa is
recently described species and endemic to
Western Ghats of India. I carried out the
study pertaining to their distribution in
Northern western Ghats i.e. Satara district.
I collected the data with the help of local
snake rescuer’s & from Non-Governmental
Organizations and showed their regional
distribution in Satara district which is a
part of Northern Western Ghats. Their
taxonomic characters are studied with the
help of museum specimen from Zoological
survey of India, Pune & Bombay Natural
History society, Mumbai. Mapped preliminary
efforts towards the mapping the distribution
of Boidae was done. Distribution was mapped
by using Q-GIS. This is first hand result
done for distribution of family Boidae in
Satara. In present study it is found that
there are few location like
Mahabaleshwar, Patan, where all three
members of family Boidae are present.
E.conicus & E.johnii these two species share
very much similar range of distribution in
Satara district. E.conicus & E.johnii are
found in following localities in Satara; Wai,
Mahabaleshwar, Patan, Koyna, Tapola, Kashil,
Bamnoli, Kusumbi, Medha, Jor. E.whitakeri is
found in following localities in Satara;
Yavteshwar, kas, Vasole, Dhom, Menavali,
Khandala, Patan, Tapola. |
Masters Dissertations – 2016
|
Project Title:
Factors governing the insect-herbivores of
Jatropha nanaDalz. &Gibs.: an endemic
species from India and Predicting the
distribution of Jatropha nanaDalz. & Gibs
through ecological niche.
Name of the student: Ashish N. Nerlekar |
|
Ecology of endemic
Jatrophas needs to be studied as desirable
wild traits in them have been proposed to be
incorporated in the biodiesel crop J. curcas
for crop improvement.Jatropha nana Dalz&Gibs.is
an endemic, threatened plant having great
economic potential, inspite of which,
insect-herbivores ofJ. nana have only been
superficially researched. Thus the present
study aims to assess the factors governing
insect- herbivore diversity on this plant,
its characteristics, temporal trends and
chief predictor variables. A two-level field
sampling was employed for the target
population on three hills in Pune city for
12 times from May-September 2015. Insect-
herbivore diversity, density of ramets,
phenology, disturbance, and climatic factors
were measured for each of the 36 clumps and
all ramets within it, which were the two
units of sampling. Through the samplings, a
total of 18 insect herbivores were reported
out of which the moth Pempelia c.f.
morosaliswas most abundant. The diversity
indices and estimators rise till sampling 10
and then fall till sampling 12. The
Michaelis-Menten equation predicted 23
insects and the rank-abundance plot
indicated an assemblage with high dominace.
A cluster analysis for all samplings
revealed that samplings 1 and 12 were most
similar to each other than rest. An
exploratory Principal Component Analysis
revealed similar patterns in similarity of
samplings and that temperature, phenology
and density were the predictor variables
that chiefly contributed to the data
variance. Several significant correlations
within the predictor variables were
observed. This is the first systematic field
study on this theme and provides baseline
data for further research on J. nana. The
data generated through this study will have
tremendous significance in the near future
and will prove useful for researchers after
J. nana is successfully hybridized with J.
curcas. Similar studies should be replicated
for other endemic Jatrophas in India. |
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Project Title:
Assessment of intra-population genetic
diversity in Ficus religiosa L. from Pune
city using ISSR markers
Name of the student: Sneha Sadanand Joshi |
|
The present study
focuses on single population, but more study
is required to be done on entire
distribution range of Ficus religiosa with
more sample size and appropriate marker in
terms of type of marker (dominant,
co-dominant), selection and number of
primers (universal and species specific)
etc. to understand complex genetic
structure. Finding of this study gives
insights of intra-population genetic
variation maintained by a well-adapted and
successful urban tree, which can be utilized
for future breeding and conservation
strategies, selection of source populations
in plantation programs and its management in
urban environment. |
|
Project Title:
Molecular phylogeography of Calotes
versicolor from the Western Ghats of India.
Name of the student: Gaurang G Gowande |
|
Calotes versicolor has
been largely neglected by taxonomists in
India and worldwide in the post-independence
period. We carried out a molecular
phylogeographic study in the Western Ghats
of India, based on the sequences obtained by
us from the Northern, Central and Southern
Western Ghats of India. Our results
demonstrate that Calotes ‘versicolor’ is not
a single lineage and also highlights the
existence of at least one undescribed taxon.
Our investigations revealed the existence of
two well-supported deeply divergent clades,
isolated spatially by the Palghat Gap to
some extent. This demonstrates that agamid
taxonomy in India is still in a primitive
state and that the agamid diversity is
largely underestimated. We propose that
Calotes ‘versicolor’ in India demands
thorough taxonomic revision and integration
of molecular phylogenetics with classical
systematic can help elucidate the systematic
and evolutionary relationships in the genus
Calotes. Besides, we also designate a
neotype for the species, as the holotype has
been lost or stolen or misplaced, and in the
absence of type material for comparison,
systematic studies would remain incomplete. |
|
Project Title:
Arachnid (Spider) diversity across various
habitat gradient of Mulshi Taluka, Pune,
Maharashtra
Name of the student: Amruta Jagdish Chavare |
|
Spiders are one of the
most diverse group of organisms they play a
major role in terrestrial ecosystem. They
exhibit a variety of foraging strategies by
which they exert a control over invertebrate
populations in varying ecological niches.
They also serve as criteria for becoming an
ecological indicator. Their high abundance,
diversity, habitat preferences, foraging
strategies, ease of collection allows the
researcher to monitor them effectively. The
aim of this study was to identify the spider
diversity present in Mulshi and its
surroundings, and to determine to what
degree of species composition varies within
habitat type. Comparisons were made between
the 3 sites on the basis of habitat and
diversity of spider was observed within the
sites. The results from this data
demonstrated large degree of variability
which correlates with habitat type. Species
abundance and diversity Shannon and Simpson
index were used to record information of
static index in Mulshi of spiders and also
to record a dominance index because it gives
more weight to common or dominant species. |
|
Project Title:
Characteristics of Vocal Signals in Sykes’s
Lark
Name of the Student: Pranjal Joshi |
|
The Sykes’s Lark (Galerida
deva) is a passerine bird of the family
Alaudidae. Larks have a melodious song,
which is often distinctive (Grimmett et al.
1998). It is endemic to India and mainly
found in Central India (Grimmett et al.
1998). Very little is known about the
characteristics of vocalizations and
behavior of the Sykes’s Lark. The focus of
my research was to document the spectrum and
analyze the characteristics of vocalizations
and possible functions associated with these
vocalizations. Also analysis of song pattern
was one of the objectives. The recordings
were done at Saswad, Pune between June and
October 2015. Behavior associated with these
calls/songs was noted. All recordings of
vocalizations were assessed using sound
analysis software (Audacity and RAVEN) to
generate sound spectrograms for comparison.
Each call/song was assessed for frequency,
call duration and frequency at maximum
amplitude measurements. For each song/call,
the song patterns were identified by
studying the pectrogram of each recording
(Catchpole & Slater, 1995). The minimum
frequency varied from 1.5-2.5 kHz, the
maximum frequency varied from 4.5-6.5 kHz
and the frequency at maximum amplitude
varied from 2-4 kHz. Each phrase from the
individual call/song was identified and
assigned a letter code. 162 unique phrases
were found in the recordings made. This
species emits a wide variety of phrases and
is a prolific singer. The Sykes’s Lark is
also a mimic and mimics many types of calls
of other birds. Two types of behaviors
associated with singing and two types of
behaviors associated with calling were
observed. Birds were observed to be singing
by hovering in the air and standing on
prominent perches like small stones. Birds
were observed to be calling while flying
short distances and in between feeding. |
|
Project Title:
The impact of tourism on herbaceous
vegetation with special focus on invasive
species of a rock outcrop in Lonavala, Pune
district, Maharashtra.
Name of the students: Noopur H. Borawake |
|
Tourism is one of the
fastest growing industries of the world.
This rapid growth of the industry has a
major impact on both people and nature.
Tourism is a double edged sword since its
effects could be both positive as well as
negative. Nature-based tourism,
agro-tourism, ecotourism and community-based
tourism are the new emerging forms of
tourism. The present study is concerned with
nature based tourism, which is a tourism
based activity in natural areas. is a
scarcity of information on the impact of
biotic pressures like tourism on rock
outcrops in India. Hence, the present study
focuses on assessing the impact of tourism
on native herbaceous vegetation and attempts
to understand the abundance of invasive
species on the rock outcrops of Lonavala. |
|
Project Title:
Collection and estimation of Non Timber
Forest Products (NTFP’s) from the dry
deciduous forest and its contribution to the
economy of tribal community.
Name of the Students: Mistry Divya U. |
|
The area at the lower
west end of the Satpura Ranges is a forest
cover with dry deciduous ecoregion that is
of due importance to the tribal communities
living there. Non Timber Forest Products are
an important source of subsistence and
income. The diversity of those forest
products in such an area with high
temperatures, their seasonal availability
and market value if any will give us the
idea of overarching projects concept. The
Socio economic factor, analysis of the
available data of the place, current debate
associated with the place, issues between
the tribal community and forest department
officials etc are things which have been
taken a note of. The community relies on
biodiversity, the problems they come across
due to external and internal threats which
affect the NTFP collection are also
surveyed. |
|
Project Title:
Preliminary scoping for birds as
‘Bioindicators’ of water quality in and
around Pune, India
Name of the student: Surabhi V Walavalkar |
|
Bird diversity and
water quality was studied in four areas of
Pune district, Maharashtra, India in late
monsoon and winter season. 80 bird species
were observed and correlated with water
quality of that particular area. Highest
Shannon and Simpson index was observed at
Mula Mutha Bird Sanctuary, Yerwada and
species are more evenly distributed at this
site. At Khadakwasla, the species are
unevenly distributed. Good water quality was
observed at Khadakwasla. It was determined
by studying few physico-chemical properties
which includes dissolved oxygen, biochemical
oxygen demand, chemical oxygen demand, pH,
temperature, alkalinity, hardness, turbidity
and total dissolved solids. This study also
provides an overview of anthropogenic
threats in the study areas. |
|
Project title:
Effect of soil texture and antlion size on
antlion pit dimensions.
Name of the Student: Arjit Jere |
|
Antlion ecology and
behavioral research is very fragmentary in
India. Significant knowledge gap exists for
study of antlion ecology in India. Basic
Understanding of behavior and ecology of
these insects will be important to ascertain
its research as well as ecological value in
future. The work aims to study if variation
in soil texture has any effect on antlion
pit dimensions like diameter and depth. It
also aims to investigate if antlion length
is correlated with pit dimensions. Both
these inquiries have been carried out
independently. |
|
Project Title: Study
of breeding biology and acoustics of the
koyna toad (Xanthophryne koynayensis)
Name of the Student: Vedant R. Dixit |
|
This species
Xanthophryne koynayensis is a toad belonging
to family Bufonidae. It is endangered
species endemic to Northern Western Ghats
and reported from in and around Koyna
Wildlife sanctuary. X.koynayensis is
strictly adapted to the laterite and breeds
only on lateritic plateaus or boulders.
Although general information on breeding
biology of the species from this genus is
known, there was no detailed study available
for this species. Aspects of breeding
biology we studied are vocalization,
amplexus, oviposition, territorial behaviour
and development of tadpoles. Here we
characterized call of X.koynayensis for the
first time and we studied few other aspects
of natural history i.e. foraging behaviour
and roosting behaviour. This is the first
basic qualitative study of X.koynayensis
breeding behaviour. |
|
Project Title:
Butterflies as bioindicators of habitat
destruction across four habitats in Northern
Western ghats, Maharashtra, India
Name of the Student: Vidula Varadarajan |
|
Climate and disturbance
is known to have a hand in shaping species
richness and number of individuals in a
given region. Past studies have shown
butterflies can act as indicator species to
reflect the health of the ecosystem. A study
was initiated to analyse how the dynamics of
butterfly population count can indicate
habitat destruction. Four study sites were
chosen on the basis of contrasting
vegetation. Number of different butterfly
species in four study sites over a period of
seven months were counted by systematic and
random sampling. Sinhagad valley is a
forest, Pachgaon Parvati and Vetal tekadi
are hillocks and Peacock bay is a grassland
valley. Highest diversity of species (59)
was recorded in Sinhagad. Disturbance was
analysed using five parameters grazing,
fire, built up, lopping and deforestation.
Each parameter was scored by cumulative
disturbance index. Habitat quality affects
butterfly species richness and composition. |
|
Project Title:
Effect of non-coated ceo2 (cerium dioxide)
and peg coated CEO2 nanoparticles on
bacterial system
Name of the Student: Priyanka Dange |
|
The emergence of
multiple, important applications for CeO2
nanoparticles (CNPs) and increased
industrial production will undoubtedly lead
to environmental release of nanoparticles.
The aim of this project was to synthesize
non-coated and PEG coated CNPs and check
their comparative effect on bacterial
system. The non-coated CNPs & PEG coated
CNPs were synthesized, dispersed and
characterized by UV. SOD mimetic activity
was observed for both noncoated and PEG
coated CNPs. Antibacterial activity was not
observed against E. coli & S.aureus at
neutral pH. Pro-oxidant and anti-oxidant
effect of PEG coated and non-coated CNPs was
observed on the bacterial system. |
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